Marja Lamoree and Timo Hamers receive STW project grant for project on High-Throughput Effect-Directed Analysis
Both IVM researchers have received this grant of the Dutch Technology Foundation STW in close collaboration with Jeroen Kool and Wilfried Niessen of the Division of BioMolecular Analysis of the VU University and Corine Houtman of The Waterlaboratory
06/19/2012 | 3:12 PM
The project, entitled “High-Throughput Effect-Directed Analysis: a novel platform for rapid and sensitive identification of toxic compounds in the aquatic environment”, focuses on the development of improved spotter technology for micro- and nanofractionation and on miniaturization of cell based assays, in order to improve the bioactivity-to-identity correlation. This project will deliver a platform capable of efficient identification of hazardous compounds in the environment that might threat human health.
All kinds of emerging contaminants, such as pharmaceuticals, endocrine disrupting and perfluorinated compounds, are present in the aquatic environment, wastewater and in drinking water sources. In addition, numerous bioassay surveys have demonstrated the presence of not yet identified compounds with toxic activities in the environment, which is evidently of great concern. Currently, identification of toxic compounds is done by effect-directed analysis (EDA). In this approach, complex environmental samples showing toxic activity are fractionated using liquid chromatography into a limited number of fractions. Each fraction is tested for toxicity in a bioassay that represents a specific toxicological endpoint. Ultimately, the “hot fractions” are analyzed with mass spectrometry to identify the compounds responsible for the observed activity. The success rate of EDA studies is rather limited: they often fail to identify the responsible toxic compounds, because, even after repeated fractionation, biologically active fractions remain too complex for chemical identification.
Contact person: Marja Lamoree